Tough Stuff Trophy
Survive the first encounter with General Glaive
You first encounter Glaive in level 12, "Glaive: 1st Encounter". Glaive is an actual boss battle, and has a white health bar above it's ship. Defeat Glaive to complete the level and unlock the trophy. You can die and still unlock the trophy, but it will ruin your "Perfect" medal run for the level.